Mr Richard Rae
Experienced working in both a small and a large biotech setting, including international work experience having worked in the UK and Germany. A native English speaker, who is speaks fluent German.
A background in Biochemistry and Immunology has enabled me to work as a scientist specialising in flow cytometry, cellular assay development, GxP work and clinical trial monitoring. I am highly driven and enthusiastic allowing me to transfer my communication skills and scientific background into any setting.
Institute for Translational Oncology (TrOn)
November 2010 – Present
Wissenschaftler, Translation Oncology, Mainz.
Deputy Head of FE-Immunomonitoring 2015-present
• Specialising in the design and optimisation of multicolour flow cytometry panels.
• Led DTH biopsy projects
• Designed optimised peptide pools for patient monitoring.
• Biomarker development.
• Performed multicolour acquisitions using the LSR Fortessa, FACSCanto II and FACSVerse.
• Responsible for monitoring of samples from cancer therapy clinical trials.
• Optimised cellular assays for NHP studies.
• Gained experience of advanced FCS file analysis using FlowJo and FACSDiva.
• German:English translation of scientific writing of journal articles and PhD theses.
• Gained experience of GxP lab requirements.
Research Assistant Cell Medica, November 2007 – November 2010 (3 years 1 month) London, United Kingdom
• Worked in both R&D and in a clinical role.
• Led Streptamer based R&D projects leading to their use in patients.
• Routinely performed multicolour flow cytometry on the BD FACSAria, FACScaliber and FACScan.
• Trained to isolate virus specific T lymphocytes from blood using the CliniMACS in a sterile environment.
• Wrote SOPs for clean room techniques.
• Fully trained to be able to perform sterile techniques in a GMP grade clean room.
• Treated patients in a phase III multicentre clinical study.
• Optimised patient monitoring using a combination of ELISPOT, immunophenotyping using flow cytometry, RNA and DNA extractions and rtPCR, validating each for use in our clinical study.
• Performed Fluorospot for two colour cytokine analysis.
• Acquainted with FACS sorting using the FACSAria
• Used PCR and gel electrophoresis for DNA analysis.
• Presented data obtained to funding bodies leading to investment.
• In charge of ordering equipment and fully stocking laboratory.
• Trained new members of staff in lab techniques
Consultant, Cell Medica, November 2006 to November 2007
• Worked on an original business plan whilst researching the viability of the market for a new cell therapy company, which is now in place.
• Wrote and presented numerous documents to possible investors and scientific persons.
• Presented data to help successfully gain funding from the Wellcome Trust as well as securing funds from other private investors.
• Researched literature regarding cell therapies and the regulation of cell therapies.
• Liased with MHRA regarding GMP and GLP standards required for a clinical trial.
Market Operator, Betfair, May 2002 to November 2006
Betfair is an internet based person to person betting exchange.
• Integrated into the team, offering leadership when required.
• Worked extensively on PCs using the internet as a resource for data.
• Trained new personnel.
2004 – 2005 Imperial College School of Medicine
6 month project in Dr Federica Marelli-Berg’s lab: The effect of PI3K delta subunit of anergy induction in murine T lymphocytes.
This project involved a great amount of cell culture work along with maintaining cell lines
Performing proliferation assays using tritiated thymidine.
Using flow cytometry to track up-regulation of activation markers
2001 – 2004 Imperial College London
6 week project in Dr David Leak’s lab: The transfection of the alkene monooxygenase operon Into Xanthobacter autotrophicus.
Carrying out polymerase chain reaction to amplify plasmid DNA.
Performing endonuclease digestions to allow insertion of genes into plasmids.
Gel electrophoresis for validation and purification of PCR products.
Transfection of E.coli and X. autotrophicus
1999 – 2001 City of Sunderland College, Bede Campus
A levels- Chemistry A, Mathematics B, Biology B. General Studies AS-Level A.
1994 – 1999 Thornhill Comprehensive School
GCSEs- 11 Grade A* – C’s. (English and Mathematics A, A)
ROLES AND ACHIEVEMENTS
Member of the CIMT.
Member of the International Society for Cellular Therapy – attending the 2009 international conference.
Attended, presented posters and written meeting reports for CIMT annual conference.
Founded and captained several sports teams.
English Native Speaker
German level B2/C1
Dr. Mark Lowdell – email@example.com
Others on request